Vaccines and Immunization

Biography

Biography: Yoshinori HAYAKAWA

Abstract

New influenza pandemic might kill 300 million people in the world. Ordinary vaccines are too costly for many people in developing countries. Moreover vaccines are not available in early stages of pandemic. Live attenuated H7N9 Influenza virus vaccine for ferrets using reverse genetics has been reported. They have used green fluorescent protein for vaccine. Ferret nasal mucosa is carcinized using carcinogen for easiness of incubation. This attenuated virus is sprayed to many cultured cancer cell specimen incubated. In some specimen attenuated virus will (mutated to) increase in cancer cells, checked by green fluorescence. Then the virus is tested to infect ferrets and then human volunteers without serious symptom. Virus with strongest virus titer to infect ferrets is selected as seed virus of live vaccine. The seed virus will be increased in incubated cancer cells by bioreactors all over the world and sprayed to vulnerable people, e.g., soldiers, students, people in slams, medical staffs, and people engaged in lifeline. Drones may be used to enhance infection, spraying in slams and markets thin capsules including the live vaccine. Thin capsules are melted at human nasal mucous membrane. Thus basic immunity is gained by many people against dangerous virus. Artificial pandemic against dangerous virus as H7N9, H5N1 etc., are to be created serially with few years interval. Artificial pandemic should be initiated before wild type pandemic starts. The reason is to avoid reassortment (mixture) of virus RNA and to avoid clinical and social confusion. It should not overlap with influenza season. The number of victims seems to be less than one thousands of wild type pandemic. The calculation assumes the number of victims with infectious attenuated live vaccine is less than that of A/H1N1. Similar technique cannot be used for creating biological weapon as toxic virus kill cancer cells for incubation.